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SRX12471938: Arima HiC of Girardinichthys multiradiatus: Adult, male, Library L45733
1 ILLUMINA (Illumina NovaSeq 6000) run: 346.6M spots, 104.7G bases, 30.7Gb downloads

Design: Hi-C confirmation capture and Illumina sequencing of a third individual was done at the DcGC by applying the Arima Genomics Hi-C kit and the library preparation using the KAPA Hyper prep kit and KAPA Library Quantification Kit (Roche Sequencing, Waltham, MA, USA). In brief, 76 mg of splitfin muscle tissue was cross-linked according to the Arima Hi-C user guide for Animal tissues (document number A160132 v01, Material Part Number: A510008). Following the large animal protocol, one third of the cross-linked tissue was digested with the Arima v1 two-enzyme cocktail, single stranded ends were filled, and these fragments were labeled with Biotin. Adjacent ends of the same cross-linked complex were ligated and purified resulting in proximity ligated DNA fragments. Shearing of the proximity ligated DNA has been done with the Covaris S2 (Covaris, Woburn, MA, USA) in Snap-Cap microTUBEs and they were selected for DNA fragments of 200-600 bps in size and finally enriched with Streptavidin-based enrichment beads. For Illumina library preparation, a modified version of the standard Kapa Hyper prep protocol (version: A160139 v00) on enrichment beads has been applied.
Submitted by: Max Planck Institute of Molecular Cell Biology and Genetics
Study: Girardinichthys multiradiatus Genome sequencing and assembly
show Abstracthide Abstract
The Darkedged Splitfin (Girardinichthys multiradiatus) is an endangered species of cold-water fish from the mountains of Central Mexico belonging to the family Goodeidae. It is live-bearing with a highly developed placenta. DNA for Pacbio sequencing and 10X sequencing was collected from mixed organ of an individual; DNA for Bionano sequencing, and liver from another individual; DNA for HiC sequencing, body muscle of the third individual. All samples were from laboratory-born descendants of fish collected by Constantino Macias Garcia of Universidad Nacional Autonoma de Mexico in 2011 at Salazar Lagoon, Mexico. The samples were prepared from the Long Read team of the DRESDEN concept Genome Center, and sequencing was conducted at DRESDEN concept Genome Center, MPI for Evolutionary Biology, and Phase Genomics. Genome assembly and manual curation of chromosomes was done by Martin Pippel of Gene Myers lab at MPI-CBG. The primary haplotype contains the longest contigs of the DAmar assembly, phased with 10X linked reads, scaffolded with Bionano optical maps, and Phase Genomics HiC reads.
Sample: Arima HiC of of Girardinichthys multiradiatus: Adult, male
SAMN22028755 • SRS10436919 • All experiments • All runs
Library:
Name: fGirMul5_HC_L45733
Instrument: Illumina NovaSeq 6000
Strategy: Hi-C
Source: GENOMIC
Selection: PCR
Layout: PAIRED
Runs: 1 run, 346.6M spots, 104.7G bases, 30.7Gb
Run# of Spots# of BasesSizePublished
SRR16187331346,552,763104.7G30.7Gb2021-10-05

ID:
16912433

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